@GoodfireAI: 3 months ago, we used interpretability to predict which of 4.2 million genetic variants cause disease. Now, we've valid…

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Summary

GoodfireAI used interpretability to predict which genetic variants cause disease, validated predictions with real-world data, and released an open-source database for all variants in the NIH's ClinVar database.

3 months ago, we used interpretability to predict which of 4.2 million genetic variants cause disease. Now, we've validated several of those predictions with real-world datasets, including a national biobank, clinical data, and RNA sequencing data. 4 examples: (1/6)
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3 months ago, we used interpretability to predict which of 4.2 million genetic variants cause disease.

Now, we’ve validated several of those predictions with real-world datasets, including a national biobank, clinical data, and RNA sequencing data.

4 examples: (1/6)

Do EVEE’s predicted pathogenic variants actually show up in human disease?

@7uomoki checked them against the FinnGen biobank, and found that they were strongly enriched for disease associations across the Finnish population—an independent validation of EVEE’s predictions. (2/6)

We also found evidence that EVEE’s pathogenicity scores track how often a variant causes disease (clinical penetrance) as part of our collaboration with @MayoClinic.

EVEE scores appear to predict the severity of familial hypercholesterolemia (FH) better than other computational predictors. (3/6)

We’ve also further validated EVEE’s mechanistic hypotheses, which predict how a variant affects downstream function.

RNA-seq data confirms many predicted effects from EVEE’s disruption profiles, including specific impacts on splicing at nucleotide-level resolution. (4/6)

Lastly, by combining EVEE’s mechanistic hypotheses with evidence from the literature, we identified 6 variants that may warrant reclassification in ClinVar (the NIH database of clinically interpreted variants).

We’ve submitted supporting evidence which is now under review. (5/6)

We’re now deploying EVEE with collaborators for clinical use cases, as well as continuing to validate more of its predictions.

Read more on how we built EVEE:

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