Machine Learning for Biomedical Raman Spectroscopy: From Spectral Acquisition to Clinical Translation

# Machine Learning for Biomedical Raman Spectroscopy: From Spectral Acquisition to Clinical Translation
Source: [https://arxiv.org/abs/2606.14169](https://arxiv.org/abs/2606.14169)
Authors:[Bogdan Oancea](https://arxiv.org/search/cs?searchtype=author&query=Oancea,+B),[Ana Maria Seciu\-Grama](https://arxiv.org/search/cs?searchtype=author&query=Seciu-Grama,+A+M),[Nicoleta Siminea](https://arxiv.org/search/cs?searchtype=author&query=Siminea,+N),[Laura Mihaela Stefan](https://arxiv.org/search/cs?searchtype=author&query=Stefan,+L+M),[Alice Stoica](https://arxiv.org/search/cs?searchtype=author&query=Stoica,+A),[Joel Sjoberg](https://arxiv.org/search/cs?searchtype=author&query=Sjoberg,+J),[Marian Necula](https://arxiv.org/search/cs?searchtype=author&query=Necula,+M),[Ana\-Maria Prelipcean](https://arxiv.org/search/cs?searchtype=author&query=Prelipcean,+A),[Corneliu Ovidiu Vrancianu](https://arxiv.org/search/cs?searchtype=author&query=Vrancianu,+C+O),[Eduard Milea](https://arxiv.org/search/cs?searchtype=author&query=Milea,+E),[Andrei Păun](https://arxiv.org/search/cs?searchtype=author&query=P%C4%83un,+A),[Ion Petre](https://arxiv.org/search/cs?searchtype=author&query=Petre,+I),[Mihaela Păun](https://arxiv.org/search/cs?searchtype=author&query=P%C4%83un,+M)

[View PDF](https://arxiv.org/pdf/2606.14169)

> Abstract:Raman spectroscopy provides label\-free, chemically specific characterization of biological systems and has become an important tool for cancer diagnosis, molecular subtyping, microbiological identification, and intraoperative decision support\. Biomedical Raman spectra are, however, high\-dimensional, noisy, and affected by fluorescence background, acquisition variability, and biological heterogeneity, making robust computational analysis essential\. This review examines the role of machine learning across the biomedical Raman spectroscopy pipeline, from preprocessing and signal correction to unsupervised structure discovery, supervised diagnosis and molecular stratification, representation and transfer learning, explainability, biomarker discovery, and multimodal integration with imaging, pathology, and molecular profiling\. Emphasis is placed on the use of machine learning not only for diagnostic classification, but also for biologically interpretable and clinically actionable analysis\. We also discuss the main barriers to clinical translation, including limited dataset sizes, inter\-instrument variability, inconsistent preprocessing, insufficient external validation, reproducibility concerns, and limited sharing of software, data, and metadata\. We argue that progress will require methodological advances together with standardization, robust validation, explainability, and deployment\-ready analytical frameworks\. By integrating methodological, biomedical, and translational perspectives, this review outlines key directions for developing reliable and clinically deployable Raman\-AI systems\.

## Submission history

From: Bogdan Oancea \[[view email](https://arxiv.org/show-email/4419fc7c/2606.14169)\] **\[v1\]**Fri, 12 Jun 2026 06:51:32 UTC \(915 KB\)